Alzheimer's disease (AD) is an irreversible and neurodegenerative disorder. Its etiology is not clear, but the involvement of genetic components plays a central role in the onset of the disease. In the present study, the expression of 10 genes (APP, PS1 and PS2, APOE, APBA2, LRP1, GRIN2B, INSR, GJB1, and IDE) involved in the main pathways related to AD were analyzed in auditory cortices and cerebellum from 29 AD patients and 29 healthy older adults. Raw analysis revealed tissue-specific changes in genes LRP1, INSR, and APP. A correlation analysis showed a significant effect also tissue-specific AD in APP, GRIN2B, INSR, and LRP1. Furthermore, the E4 allele of the APOE gene revealed a significant correlation with change expression tissue-specific in ABPA2, APP, GRIN2B, LRP1, and INSR genes. To assess the existence of a correction between changes in target gene expression and a probability of AD in each tissue (auditory cortices and cerebellum) an analysis of the effect of expressions was realized and showed that the reduction in the expression of the APP in auditory cortex and GRIN2B cerebellum had a significant effect in increasing the probability of AD, in the same logic, our result also suggesting that increased expression of the LRP1 and INSR genes had a significant effect on increasing the probability of AD. Our results showed tissue-specific gene expression alterations associated with AD and certainly opened new perspectives to characterize factors involved in gene regulation and to obtain possible biomarkers for AD.
Alzheimer Disease , Antigens, CD , Low Density Lipoprotein Receptor-Related Protein-1 , Humans , Alzheimer Disease/genetics , Alzheimer Disease/metabolism , Male , Female , Aged , Low Density Lipoprotein Receptor-Related Protein-1/genetics , Cerebellum/metabolism , Receptors, N-Methyl-D-Aspartate/genetics , Receptor, Insulin/genetics , Receptor, Insulin/metabolism , Auditory Cortex/metabolism , Amyloid beta-Protein Precursor/genetics , Aged, 80 and over , Apolipoproteins E/genetics , Gene Expression/genetics , Case-Control Studies
NTRODUCTION: Type 2 Diabetes Mellitus (DM2) can lead to autonomic nervous system dysfunction and heart rate variability (HRV) is often used to assess this system both during rest and during physical exercise. OBJECTIVE: To evaluate the autonomic modulation at rest and the responses of heart rate and parasympathetic indices of individuals with DM2 to isometric handgrip exercise. METHOD: The sample consisted of individuals of both sexes; over 40 years, divided into groups, with and without DM2 diagnosis. The collection of resting HRV was performed for 20 minutes in the supine position, and 256 points were selected for symbolic and linear analysis. The individuals performed isometric contraction for one minute with intensities of 10, 20, 30, 40 and 50 % of the maximum contraction, using the parasympathetic indexes RMSSD and SD1 for analysis. RESULTS: Linear and symbolic indices of HRV at rest and those obtained during exercise were similar (p-value>0.05). No association was found between the indices at rest and the responses to exercise, with a slight delay in the response of diabetics in HR and parasympathetic indices being identified. CONCLUSION: There was no difference between the groups in the modulation of rest or in the parasympathetic adjustments of the exercise (Tab. 4, Ref. 32).
Determinar as principais causas de absenteísmo por problemas de saúde em Agentes de Segurança Penitenciária (ASPs) e relacioná-las com o envelhecimento. Estudo quantitativo, observacional e transversal, por meio de análise documental, realizado em penitenciárias masculina e feminina no interior paulista. Os dados foram analisados no software SPSS com significância de 5%. A amostra foi constituída de 79 ASPs com média de idade de 41,3 anos, tempo médio trabalhado de 11,5 anos e prevalência de absenteísmo de 18,3%. As principais causas de absenteísmo foram relacionadas a quadros emocionais, problemas ortopédicos e distúrbios gastrintestinais. Não se encontrou correlação entre idade cronológica e quantidade de afastamentos. Os dados obtidos muito se assemelham a outros estudos nacionais e internacionais, entretanto há escassez de evidências científicas abordando essa classe de trabalhadores, que carece de melhorias no cotidiano de seu exercício profissional, a fim de que desempenhe suas funções com maior dignidade.
To determine the main causes of absenteeism due to health problems in Penitentiary Security Agents (ASPs) and to relate them to aging. Quantitative, observational and cross-sectional study, based on document analysis, carried out in male and female penitentiaries in the interior of São Paulo. Data were analyzed in SPSS software with a significance of 5%. The sample consisted of 79 ASPs; with a mean age of 41.3 years; mean time worked of 11.5 years and prevalence of absenteeism of 18.3%. The main causes of absenteeism were related to emotional conditions, orthopedic problems and gastrointestinal disorders. We did not find a correlation between chronological age and number of leaves. The data obtained are very similar to other national and international studies, however, there is a lack of scientific evidence addressing this class of workers, who need improvements in the daily routine of their professional practice, in order for them to perform their duties with greater dignity.
